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Multilevel thresholding for segmentation is an essential task and indispensable process in various applications. Conventional color multilevel thresholding based image segmentations are computationally expensive, and lack accuracy and stability. To address this issue, this paper introduces the comparative performance study of different objective functions using cuckoo search and other optimization algorithms to solve the color image segmentation problem via multilevel thresholding. During the optimization process, solutions are evaluated using Otsu or Kapur's method. Performance of the proposed approach has been assessed using a variety of benchmark images, and compared against three other nature inspired algorithms namely differential evolution (DE), wind driven optimization (WDO) and particle swam optimization (PSO) algorithms. Results have been analyzed both qualitatively and quantitatively …