查看文章

A Disintegrin and Metalloproteinase (ADAM) proteins are a family of multifunctional proteins containing disintegrin and metalloproteinase domains that perform both adhesive and proteolytic functions in cell–cell and cell–matrix interactions. ADAM15 is unique among these proteins in having an Arg–Gly–Asp (RGD) motif in its disintegrin-like domain. This motif is known to interact with the integrin αIIbβ3 on platelets.

We cloned and expressed the human ADAM15 disintegrin-like domain and its derivatives in Pichia pastoris, and purified them by chromatographic fractionation. We then characterized the integrin binding specificities and their antiplatelet activities of the proteins. Antiplatelet function was assessed by inhibition of platelet adhesion and aggregation.

The yeast-expressed ADAM15 disintegrin-like domains were able to inhibit the binding of αIIbβ3 as …