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This study examines quality of academic worklife in Czech public universities to assess the extent to which the global drive towards marketisation in higher education has affected Czech academic staff. A total of 2229 academics (men = 57.1%) completed a survey measuring their job satisfaction, job stress, and work environment perceptions. Findings revealed high levels of overall job satisfaction (83.6% satisfied with their jobs) and relatively low levels of stress (13.7% regularly stressed). Most academics reported positive features of their work environment including autonomy and quality, role clarity, influence over academic work, and a strong social community. Negative features included dissatisfaction with pay, poor leadership, and pressure to produce. Job satisfaction was significantly associated with traditional academic values (focus on quality, involvement in decision-making, commitment to the workplace …