作者
Felix Krueger, Benjamin Kreck, Andre Franke, Simon R Andrews
发表日期
2012/2
来源
Nature methods
卷号
9
期号
2
页码范围
145-151
出版商
Nature Publishing Group US
简介
Bisulfite conversion of genomic DNA combined with next-generation sequencing (BS-seq) is widely used to measure the methylation state of a whole genome, the methylome, at single-base resolution. However, analysis of BS-seq data still poses a considerable challenge. Here we summarize the challenges of BS-seq mapping as they apply to both base and color-space data. We also explore the effect of sequencing errors and contaminants on inferred methylation levels and recommend the most appropriate way to analyze this type of data.
学术搜索中的文章
F Krueger, B Kreck, A Franke, SR Andrews - Nature methods, 2012