作者
Hin Fung Tsang, Allen Chi Shing Yu, Nana Jin, Aldrin Kay Yuen Yim, Wai Ming Stanley Leung, Ka Wai Lam, William Chi Shing Cho, Jiachi Chiou, Sze Chuen Cesar Wong
发表日期
2022/5/4
来源
Expert Review of Molecular Diagnostics
卷号
22
期号
5
页码范围
575-582
出版商
Taylor & Francis
简介
Introduction
Clinical metagenomic next-generation sequencing (mNGS) allows a comprehensive genetic analysis of microbial materials. Different from other traditional target-driven molecular diagnostic tests, such as PCR, mNGS is a hypothesis-free diagnostic approach that allows a comprehensive genetic analysis of the clinical specimens that cover nearly any common, rare, and new pathogens ranging broadly from viruses, bacteria, fungi to parasites.
Areas covered
In this article, we discussed the clinical application of the mNGS using two clinical cases as examples and described the use of mNGS to assist the diagnosis of parasitic pulmonary infection. The advantages and challenges in implementing mNGS in clinical microbiology are also discussed.
Expert opinion
mNGS is a promising technology that allows quick diagnosis of infectious diseases. Currently, a plethora of sequencing and analysis methods exists …
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HF Tsang, ACS Yu, N Jin, AKY Yim, WMS Leung… - Expert Review of Molecular Diagnostics, 2022