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Estimation/updating of Origin–Destination (OD) flows and other traffic state parameters is a classical, widely adopted procedure in transport engineering, both in off-line and in on-line contexts. Notwithstanding numerous approaches proposed in the literature, there is still room for considerable improvements, also leveraging the unprecedented opportunity offered by information and communication technologies and big data. A key issue relates to the unobservability of OD flows in real networks – except from closed highway systems – thus leading to inherent difficulties in measuring performance of OD flows estimation/updating methods and algorithms. Starting from these premises, the paper proposes a common evaluation and benchmarking framework, providing a synthetic test bed, which enables implementation and comparison of OD estimation/updating algorithms and methodologies under “standardized …