作者
Xuping Xie, Antonio Muruato, Kumari G Lokugamage, Krishna Narayanan, Xianwen Zhang, Jing Zou, Jianying Liu, Craig Schindewolf, Nathen E Bopp, Patricia V Aguilar, Kenneth S Plante, Scott C Weaver, Shinji Makino, James W LeDuc, Vineet D Menachery, Pei-Yong Shi
发表日期
2020/5/13
期刊
Cell host & microbe
卷号
27
期号
5
页码范围
841-848. e3
出版商
Elsevier
简介
The ongoing pandemic of COVID-19, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), underscores the urgency to develop experimental systems for studying this virus and identifying countermeasures. We report a reverse genetic system for SARS-CoV-2. Seven complimentary DNA (cDNA) fragments spanning the SARS-CoV-2 genome were assembled into a full-genome cDNA. RNA transcribed from the full-genome cDNA was highly infectious after electroporation into cells, producing 2.9 × 106 plaque-forming unit (PFU)/mL of virus. Compared with a clinical isolate, the infectious-clone-derived SARS-CoV-2 (icSARS-CoV-2) exhibited similar plaque morphology, viral RNA profile, and replication kinetics. Additionally, icSARS-CoV-2 retained engineered molecular markers and did not acquire other mutations. We generated a stable mNeonGreen SARS-CoV-2 (icSARS-CoV-2-mNG) by …
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X Xie, A Muruato, KG Lokugamage, K Narayanan… - Cell host & microbe, 2020