作者
Saurabh Awasthi, Pongsatorn Sriboonpeng, Cuifeng Ying, Jared Houghtaling, Ivan Shorubalko, Sanjin Marion, Sebastian James Davis, Laura Sola, Marcella Chiari, Aleksandra Radenovic, Michael Mayer
发表日期
2020/11
期刊
Small Methods
卷号
4
期号
11
页码范围
2000177
简介
Nanopore‐based resistive‐pulse recordings represent a promising approach for single‐molecule biophysics with applications ranging from rapid DNA and RNA sequencing to “fingerprinting” proteins. Based on advances in fabrication methods, solid‐state nanopores are increasingly providing an alternative to proteinaceous nanopores from living organisms; their widespread adoption is, however, slowed by nonspecific interactions between biomolecules and pore walls, which can cause artifacts and pore clogging. Although efforts to minimize these interactions by tailoring surface chemistry using various physisorbed or chemisorbed coatings have made progress, a straightforward, robust, and effective coating method is needed to improve the robustness of nanopore recordings. Here, covalently attached nanopore surface coatings are prepared from three different polymers using a straightforward “dip and rinse …
引用总数
20202021202220232024261177
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