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Tree species composition of forest stand is an important indicator of forest inventory attributes for assessing ecosystem health, understanding successional processes, and digitally displaying forest biodiversity. In this study, we acquired high spatial resolution multispectral and RGB imagery over a subtropical natural forest in southwest China using a fixed-wing UAV system. Digital aerial photogrammetric (DAP) technique was used to generate multi-spectral and RGB derived point clouds, upon which individual tree crown (ITC) delineation algorithms and a machine learning classifier were used to identify dominant tree species. To do so, the structure-from-motion method was used to generate RGB imagery-based DAP point clouds. Then, three ITC delineation algorithms (i.e., point cloud segmentation (PCS), image-based multiresolution segmentation (IMRS), and advanced multiresolution segmentation (AMRS …