作者
Fahumiya Samad, Koji Yamamoto, Manjula Pandey, David J Loskutoff
发表日期
1997/1
期刊
Molecular medicine
卷号
3
页码范围
37-48
出版商
BioMed Central
简介
Background
Tumor necrosis factor-α (TNF-α) is chronically elevated in the adipose tissue from obese humans and mice. This increase in TNF-α contributes to the insulin resistance, elevated plasminogen activator inhibitor-1 (PAI-1) levels, and cardiovascular complications associated with obesity and noninsulin-dependent diabetes (NIDDM). PAI-1 gene expression in adipose tissue is also stimulated by transforming growth factor-β (TGF-β). Experiments were performed to determine whether TGF-β is regulated by TNF-α and elevated in obesity.
Materials and Methods
The concentration of TGF-β and PAI-1 mRNA in murine adipose tissue and cultured 3T3-L1 adipocytes was determined by quantitative reverse transcription polymerase chain reaction (RT-PCR), and the cellular localization of these molecules was evaluated using in situ hybridization and cell …
引用总数
学术搜索中的文章
F Samad, K Yamamoto, M Pandey, DJ Loskutoff - Molecular medicine, 1997