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A nutritionally induced obesity model was used to investigate the modulation of fibrinolytic and gelatinolytic activity during the development of adipose tissue.

Five week old male mice were fed a standard fat diet (SFD, 13% kcal as fat) or a high fat diet (HFD, 42% kcal as fat) for up to 15 weeks. The HFD resulted in body weights of 31 ± 0.9 g, 38 ± 2.0 g and 47 ± 1.9 g at 5, 10 and 15 weeks, respectively; corresponding values for mice on the SFD were 26 ± 0.6 g, 31 ± 0.9 g and 31 ± 1.2 g (all p < 0.001). The weight of the isolated subcutaneous (SC) or gonadal (GON) fat after 15 weeks of HFD was 1,870 ± 180 mg or 1,470 ± 160 mg, as compared to 250 ± 58 mg or 350 ± 71 mg for the SFD (p < 0.001). The HFD induced marked time-dependent hyperglycemia and elevated levels of triglycerides and total cholesterol. The HFD diet also induced a marked hypertrophy of the adipocytes as compared to the SFD, e.g …