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The goal of the study was to assess the culture method and spirulina (Spirulina platensis) production in the supernatant of three distinct digested rotting ladies fingers, using Kosaric medium (KM) as the control. Two hundred gram rotten ladies finger was allowed to digestion in 4.0 L distilled water using aeration in 5.0 L glass jar. After 30 days, almost colorless supernatant was found, screened through 300 µm net and added with 9 g/l NaHCO3, 0.20 g/l urea and 0.50 ml/L micro nutrients. Three different concentrations such as 25, 50 and 75% of supernatant with three replications were prepared. Next, three digested rotten