查看文章

To obtain a systems-level understanding of Shewanella biology and ecology, the influence of electron acceptor availability on Shewanella’s growth, metabolism, and transport needs to be elucidated. The diffusion gradient chamber (DGC) is an experimental tool developed to study population-level microbial growth and motility in response to concentration gradients. In this paper, the response of populations of Shewanella oneidensis MR-1 cells to an applied single gradient of the electron acceptor fumarate and applied opposing gradients of fumarate and nitrate, also an electron acceptor, were studied in the DGC. Mathematical models capable of predicting cellular growth and chemotaxis under the influence of gradients were used to analyze the results. Examining wild-type cells grown in a single gradient of fumarate, we found that MR-1 cells formed a chemotactic band that migrated up the electron acceptor …