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In today’s world, most of the things are done online such as online banking, food ordering, car booking, and shopping. It is very difficult to protect the data utilizing the conventional cryptographic and Steganographic approach with the present techniques. DNA cryptography is one of the new areas of information security. DNA cryptography can encrypt the data or information in the form of DNA nucleotides (A, T, G and C). In this DNA Cryptography process, the data may be in the form of Audio, video, Image, or text file. It can create utilization of the huge DNA storage capacity and its biological properties like robustness, vastly constant molecule, efficient of cost and simply obtainable. However, the message encryption algorithm contains various steps to break and to obtain the original message. Therefore, in this paper, presents a Trellis algorithm with DNA sequence and random key generation to provide more security …