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Raman micro-spectroscopy and different approaches for multivariate analysis were used for an investigation of subcellular regions of X-ray exposed single SH-SY5Y human neuroblastoma cells. Nucleus and cytoplasm regions of single cells were investigated after X-rays irradiation (0, 2, 4, 6 and 8 Gy). Cells fixed immediately after irradiation and 24h irradiation were considered. Principal component analysis (PCA) and interval-PCA (i-PCA) were used for analyzing the spectra in order to highlight the changes due to the different treatments. Biochemical changes occurring in the nucleus and cytoplasm regions of single cells upon X-ray irradiation were observed. The analysis of Raman spectra allowed us to detect modifications in the contribution from proteins, nucleic acids, lipids, and carbohydrates of cells, induced at different extent on the two cell regions. The biochemical changes occurring in these cells were …