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Plant polyphenols are important components of human diet and a number of them are considered to possess chemo-preventive and therapeutic properties against cancer. They are recognized as naturally occurring antioxidants, but also as pro-oxidant, pro-apoptotic, or chromosomal aberrations inducers, depending on their concentration and/or the stage of cell-cycle of the cells with which they interact. For these reasons, particular interest is devoted to knowing the total effects of polyphenols on the cell cycle and metabolism. Fourier-Transform Infrared (FT-IR) spectroscopy thanks to its ability in analyzing cells at a molecular level can be particularly useful in investigating the biochemical changes induced in protein, nucleic acid, lipid, and carbohydrate content of cells by means of polyphenols administration. Spectroscopic analysis was performed on in vitro human SH-SY5Y neuroblastoma cells that were exposed to different doses of a cherry derived polyphenol extract. The infrared spectra that were obtained from unexposed and exposed cells show significant differences that can be helpful in order to understand the cells-polyphenols interaction.