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Methods: Raman micro-spectroscopy was performed on in-vitro single cells after irradiation by graded X-ray doses (2, 4, 6, 8 Gy). Spectra from nucleus and cytoplasm regions were acquired and the difference spectra were examined by interval Principal Component Analysis (i-PCA).

Results: The analysis by i-PCA of the nucleus-cytoplasm difference spectra allowed us to shed light on the modifications, due to X-ray irradiation, of Raman features due to components with different relative contents in the two regions or modes with a low intensity that are not appreciable in the simple spectra [3]. In particular, the effects on DNA/RNA backbone and single nucleobases and those occurring on lipids and proteins are discussed.

Conclusion: The proposed approach allowed us to highlight newly identified specific characteristics not previously reported.