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We examined the role of cytosolic phospholipase A 2 (cPLA 2) during human eosinophil adherence to ICAM-1-or VCAM-1-coated plates. IL-5-stimulated eosinophils adhered to ICAM-1 through the β 2 integrin CD11b/CD18, while nonstimulated eosinophils did not. By contrast, nonstimulated eosinophils adhered to VCAM-1 through the β 1-integrin VLA-4/CD29. Both IL-5-induced adhesion to ICAM-1 and spontaneous adhesion to VCAM-1 corresponded temporally to cPLA 2 phosphorylation, which accompanied enhanced catalytic activity of cPLA 2. The structurally unrelated cPLA 2 inhibitors, arachidonyl trifluoromethylketone and surfactin, significantly inhibited eosinophil adhesion to ICAM-1 and VCAM-1 in a concentration-dependent manner. Inhibition of secretory PLA 2, 5-lipoxygenase, or cyclooxygenase did not affect eosinophil adhesion. Addition of arachidonic acid to eosinophils after cPLA 2 inhibition with …