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Planar laser induced fluorescence (PLIF) and digital particle image velocimetry (DPIV) combined with Lagrangian coherent structure (LCS) techniques are utilized to measure ambient fluid entrainment during laminar vortex ring formation and relate it to the total entrained volume after formation is complete. Vortex rings are generated mechanically with a piston-cylinder mechanism for a jet Reynolds number of 1,000, stroke ratios of 0.5, 1.0 and 2.0, and three velocity programs (Trapezoidal, triangular negative and positive sloping velocity programs). The quantitative observations of PLIF agree with both the total ring volume and entrainment rate measurements obtained from the DPIV/LCS hybrid method for the jet Reynolds number of 1,000, trapezoidal velocity program and stroke ratio of 2.0 case. In addition to increased entrainment at smaller stroke ratios observed by others, the PLIF results also show that a …