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Ensuring security for business becomes especially relevant in the context of geopolitical conflicts, increasing digitization and introducing new technologies into the financial and production sectors, booming frequency and complexity of cyber-attacks aimed at the business sphere, as well as with more diverse applications of artificial intelligence. The article studies the specifics of ensuring business security in Ukraine during wartime using artificial intelligence and cyber protection. A retrospective review of the literature has been conducted revealing that there is no research on the ways artificial intelligence is applied to ensure business activity security, which indicates the research novelty of this article. The authors outline the potential and threats of artificial intelligence for business security. Directions for securing entrepreneurship in war conditions with the help of artificial intelligence and elimination/minimization of cyber threats are formulated. The regulatory and legislative framework governing the regulation of cyber security in Ukraine is outlined and directions for its improvement are suggested.