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Urban design significantly impacts sustainability, particularly in the context of public transit efficiency and carbon emissions reduction. This study explores two neighborhoods with distinct urban designs: South End, Charlotte, NC, featuring a dynamic mixed-use urban design pattern, and Avondale, Chattanooga, TN, with a residential suburban grid layout. Using the TRANSIT-GYM tool, we assess the impact of increased bus utilization in these different urban settings on traffic and CO2 emissions. Our results highlight the critical role of urban design and planning in transit system efficiency. In South End, the mixed-use design led to more substantial emission reductions, indicating that urban layout can significantly influence public transit outcomes. Tailored strategies that consider the unique urban design elements are essential for climate resilience. Notably, doubling bus utilization decreased daily emissions by 10.18 …