作者
Joyce Oloaigbe Ogidigo, Chioma Assumpta Anosike, Parker Elijah Joshua, Collins U Ibeji, Daniel Emmanuel Ekpo, Bennett C Nwanguma, Okwesili Fred Chiletugo Nwodo
发表日期
2021/1/1
期刊
Pharmaceutical Biology
卷号
59
期号
1
页码范围
442-454
出版商
Taylor & Francis
简介
Context
Bryophyllum pinnatum (Lam.) Oken (Crassulaceae) is used traditionally to treat many ailments.
Objectives
This study characterizes the constituents of B. pinnatum flavonoid-rich fraction (BPFRF) and investigates their antioxidant and anticholinesterase activity using in vitro and in silico approaches.
Materials and methods
Methanol extract of B. pinnatum leaves was partitioned to yield the ethyl acetate fraction. BPFRF was isolated from the ethyl acetate fraction and purified. The constituent flavonoids were structurally characterized using UPLC-PDA-MS2. Antioxidant activity (DPPH), Fe2+-induced lipid peroxidation (LP) and anticholinesterase activity (Ellman’s method) of the BPFRF and standards (ascorbic acid and rivastigmine) across a concentration range of 3.125–100 μg/mL were evaluated in vitro for 4 months. Molecular docking was performed to give insight into the binding potentials of BPFRF …
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JO Ogidigo, CA Anosike, PE Joshua, CU Ibeji, DE Ekpo… - Pharmaceutical Biology, 2021