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The aim of this paper is to assess whether objective time-course physiological responses from electroencephalogram (EEG) and electrocardiogram (ECG) can quantify stress level or not. Physiological signals were measured under emotional stress tasks using audio-visual stimuli and analyzed with a discrete Fourier transform (DFT). The results showed that beta activities in the frontal and temporal areas of the Non-Stress Group were significantly larger than those of the Stress Group under emotionally unpleasant stimuli. Also, the beta activities of the Non-Stress Group decreased with time. These events suggest that people with less stress have a high stress-resistance potential in their brain activities, which can be seen as general biological reactions.