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Background and purpose

Blood culture is important for the diagnosis of sepsis, but it is sometimes difficult to differentiate true bacteremia from pseudobacteremia. This study proposed clinical criteria and evaluated the role of repeat blood cultures in assessing the clinical significance of potential contaminants in blood cultures (PCBCs).

Methods

From February to May in 2004 (prospective study) and 2003 (retrospective study), adult patients with growth of coagulase-negative staphylococci, Bacillus spp., Micrococcus spp., Propionibacterium spp., Gram-positive bacilli, or Clostridium perfringens, collectively referred to as" PCBCs", in at least 1 set of blood cultures in a medical center were included. The demographic and clinical data of patients with PCBCs were collected, and proposed clinical criteria for true bloodstream infections were used to evaluate their clinical outcome. Also, the potential role of repeating blood cultures to differentiate true bacteremia from pseudobacteremia was evaluated.

Results

There were 212 cases with 214 PCBCs, of which coagulase-negative staphylococci predominated (182 isolates, 85.0%). The overall contamination rate was 83.9%(178/212). Repeating 2 sets of blood cultures might be useful in the clinical differentiation of true bacteremia and pseudobacteremia, since the contamination rate of patients with potential contaminants in 1 set of blood cultures declined from 95% to 87%(p= 0.04) with such a strategy. Those with true bloodstream infections had a significantly higher all-cause mortality rate at 14 days than those with pseudobacteremia (23.8% vs 7.3%, p= 0.028), suggesting the validity of the clinical criteria. Of …