作者
Sharat C Raju, Samantha J Hauff, Aaron J Lemieux, Ryan K Orosco, Andrew M Gross, Linda T Nguyen, Elamprakash Savariar, William Moss, Michael Whitney, Ezra E Cohen, Scott M Lippman, Roger Y Tsien, Trey Ideker, Sunil J Advani, Quyen T Nguyen
发表日期
2015/5/1
期刊
Oral oncology
卷号
51
期号
5
页码范围
470-475
出版商
Pergamon
简介
Objective
Patients with head and neck squamous cell carcinoma (HNSCC) containing TP53 mutation and 3p deletion (“double-hit”) have poorer prognosis compared to patients with either event alone (“single-hit”). The etiology for worse clinical outcomes in patients with “double-hit” cancers is unclear. We compared radiosensitivity of cell lines containing both TP53 mutations and deletion of Fragile Histidine Triad (FHIT, the gene most commonly associated with 3p deletion) to “single-hit” lines with only TP53 mutation. We compared radiosensitivity in a “single-hit” cell line with TP53 mutation converted to “double-hit” using RNA interference targeting FHIT. Finally, we compared matrixmetalloproteinase-2/9 (MMP-2/9) activity, a previously-established biomarker for tumor aggressiveness, in xenograft tumors derived from these cell lines.
Materials/methods
TP53 mutation and FHIT deletion profiles of HNSCC lines were …
学术搜索中的文章
SC Raju, SJ Hauff, AJ Lemieux, RK Orosco, AM Gross… - Oral oncology, 2015