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Speech separation is a process of segregating the target speech from the noisy mixture. Human auditory system naturally has the capability to separate the speech from background noise; however the machine implementation of the same has been failed. Computational Auditory Scene Analysis (CASA) is a recent approach which models the human auditory system by computational means to separate the target speech from the noisy mixture. Most of the speech separation system based on CASA uses energy as one of the reliable feature to segregate the target speech from the noisy mixture. This research work proposes a new approach using adaptive energy selection threshold to segregate the target speech. The experimental result shows significant improvement in the signal to noise ratio as compared to the conventional energy threshold method.