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The influence of relative humidity (RH) on quasistatic current-voltage characteristics of Bifidobacterium animalis subsp. lactis BB-12 thin layers have been studied for the first time. The value of electrical conductivity in 75 RH was found to be in the order of 10 (ohm cm) which was 10 orders of magnitude higher than that observed in dry atmosphere. Here we also demonstrated that RH played a key role in hysteresis behaviour of the measured characteristics. FTIR measurements showed that under water moisture environment the associated bonds for amine and carboxyl group were greatly strengthened that was the source of number of free charge carries after ionization. The type of surface charge of Bifidobacterium animalis subsp. lactis BB-12 was found to be negative by zeta potential measurements, claiming that electrons were the charge carriers.