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A novel photonic crystal fiber (PCF) biosensor based on surface plasmon resonance (SPR) phenomena is proposed. Chemically stable active plasmonic material gold (Au) and sensing layer is used outside the fiber structure to make the sensor configuration simpler. The proposed sensor would be able to detect the unknown analytes by flowing through the metal surface or dripped on the outer surface of the metal layer. The proposed sensor is consist with the symmetrical circular air-holes. Two small air-holes are used in the second rings which helps to produce the more evanescent field as well as helps to tune the phase matching between the core guided mode and surface plasmon polaritons (SPP) mode. Surface plasmons along the metal surface is excited with the leaky Gaussian-like core guided mode. Guiding properties and the sensing performance are numerically investigated by the Finite Element Method …