作者
Xiaohong Wang, Haitao Gu, Dongze Qin, Liwang Yang, Wei Huang, Kobina Essandoh, Yigang Wang, Charles C Caldwell, Tianqing Peng, Basilia Zingarelli, Guo-Chang Fan
发表日期
2015/9/8
期刊
Scientific reports
卷号
5
期号
1
页码范围
13721
出版商
Nature Publishing Group UK
简介
Mesenchymal stem cells (MSCs) have been shown to elicit cardio-protective effects in sepsis. However, the underlying mechanism remains obscure. While recent studies have indicated that miR-223 is highly enriched in MSC-derived exosomes, whether exosomal miR-223 contributes to MSC-mediated cardio-protection in sepsis is unknown. In this study, loss-of-function approach was utilized and sepsis was induced by cecal ligation and puncture (CLP). We observed that injection of miR-223-KO MSCs at 1 h post-CLP did not confer protection against CLP-triggered cardiac dysfunction, apoptosis and inflammatory response. However, WT-MSCs were able to provide protection which was associated with exosome release. Next, treatment of CLP mice with exosomes released from miR-223-KO MSCs significantly exaggerated sepsis-induced injury. Conversely, WT-MSC-derived-exosomes displayed protective …
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X Wang, H Gu, D Qin, L Yang, W Huang, K Essandoh… - Scientific reports, 2015