作者
Xiaoxuan Xia, William Ka Kei Wu, Sunny Hei Wong, Dabin Liu, Thomas Ngai Yeung Kwong, Geicho Nakatsu, Pearlly S Yan, Yu-Ming Chuang, Michael Wing-Yan Chan, Olabisi Oluwabukola Coker, Zigui Chen, Yun Kit Yeoh, Liuyang Zhao, Xiansong Wang, Wing Yin Cheng, Matthew Tak Vai Chan, Paul Kay Sheung Chan, Joseph Jao Yiu Sung, Maggie Haitian Wang, Jun Yu
发表日期
2020/12
期刊
Microbiome
卷号
8
页码范围
1-13
出版商
BioMed Central
简介
Background
Altered microbiome composition and aberrant promoter hypermethylation of tumor suppressor genes (TSGs) are two important hallmarks of colorectal cancer (CRC). Here we performed concurrent 16S rRNA gene sequencing and methyl-CpG binding domain-based capture sequencing in 33 tissue biopsies (5 normal colonic mucosa tissues, 4 pairs of adenoma and adenoma-adjacent tissues, and 10 pairs of CRC and CRC-adjacent tissues) to identify significant associations between TSG promoter hypermethylation and CRC-associated bacteria, followed by functional validation of the methylation-associated bacteria.
Results
Fusobacterium nucleatum and Hungatella hathewayi were identified as the top two methylation-regulating bacteria. Targeted analysis on bona fide TSGs revealed that H. hathewayi and Streptococcus spp. significantly correlated with CDX2 and MLH1 promoter hypermethylation …
学术搜索中的文章
X Xia, WKK Wu, SH Wong, D Liu, TNY Kwong… - Microbiome, 2020