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Methods

We used data from a randomized controlled trial (RCT) with MDD patients (N= 112) receiving a cognitive intervention. At baseline and 8 weeks, blood for DNA methylation (Illumina Infinium MethylationEPIC 850k BeadChip) was collected, as well as MADRS. First, responders (N= 24; MADRS-reduction of at least 50%) were compared with non-responders (N= 60). Then, we performed longitudinal within-individual analyses, for response (N= 21) and for remission (N= 18; MADRS smaller or equal to 9 and higher than 9 at baseline), respectively, as well as patients with no change in MADRS over time. At 8 weeks the sample comprised 84 individuals; 73 patients had DNA methylation for both time-points. The RnBeads package (R) was used for data cleaning, quality control, and differential DNA-methylation (limma). The within-individual paired longitudinal analysis was performed using Welch’s t-test …