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Self-interference (SI) is the key issue that prevents in-band full-duplex (IBFD) communications from being practical. Analog multi-tap adaptive filter is an efficient structure to cancel SI since it can capture the nonlinear components and noise in the transmitted signal. Analog least mean square (ALMS) loop is a simple adaptive filter that can be implemented by purely analog means to sufficiently mitigate SI. Comprehensive analyses on the behaviors of the ALMS loop have been published in the literature. This paper proposes a practical structure and presents an implementation of the ALMS loop. By employing off-the-shelf components, a prototype of the ALMS loop including two taps is implemented for an IBFD system operating at the carrier frequency of 2.4 GHz. The prototype is firstly evaluated in a single carrier signaling IBFD system with 20 MHz and 50 MHz bandwidths, respectively. Measured results show that the ALMS loop can provide 39 dB and 33 dB of SI cancellation in the radio frequency domain for the two bandwidths, respectively. Furthermore, the impact of the roll-off factor of the pulse shaping filter on the SI cancellation level provided by the prototype is presented. Finally, the experiment with multicarrier signaling shows that the performance of the ALMS loop is the same as that in the single carrier system. These experimental results validate the theoretical analyses presented in our previous publications on the ALMS loop behaviors.