作者
Andre Kleensang, Marguerite M Vantangoli, Shelly Odwin-DaCosta, Melvin E Andersen, Kim Boekelheide, Mounir Bouhifd, Albert J Fornace, Heng-Hong Li, Carolina B Livi, Samantha Madnick, Alexandra Maertens, Michael Rosenberg, James D Yager, Liang Zhao, Thomas Hartung
发表日期
2016/7/26
期刊
Scientific reports
卷号
6
期号
1
页码范围
1-11
出版商
Nature Publishing Group
简介
Common recommendations for cell line authentication, annotation and quality control fall short addressing genetic heterogeneity. Within the Human Toxome Project, we demonstrate that there can be marked cellular and phenotypic heterogeneity in a single batch of the human breast adenocarcinoma cell line MCF-7 obtained directly from a cell bank that are invisible with the usual cell authentication by short tandem repeat (STR) markers. STR profiling just fulfills the purpose of authentication testing, which is to detect significant cross-contamination and cell line misidentification. Heterogeneity needs to be examined using additional methods. This heterogeneity can have serious consequences for reproducibility of experiments as shown by morphology, estrogenic growth dose-response, whole genome gene expression and untargeted mass-spectroscopy metabolomics for MCF-7 cells. Using Comparative Genomic …
学术搜索中的文章
A Kleensang, MM Vantangoli, S Odwin-DaCosta… - Scientific reports, 2016