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Dendritic cells (DCs) are prime targets for vaccination and immunotherapy. However, limited control over antigen presentation at a desired maturation status in these plastic materials remains a fundamental challenge in efficiently orchestrating a controlled immune response. DC‐derived extracellular vesicles (EVs) can overcome some of these issues, but have significant production challenges. Herein, this work employs a unique chemicallyinduced method for production of DC‐derived extracellular blebs (DC‐EBs) that overcome the barriers of DC and DC‐derived EV vaccines. DC‐EBs are molecular snapshots of DCs in time, cell‐like particles with fixed stimulatory profiles for controlled immune signaling. DC‐EBs are produced in an order of magnitude more quickly and efficiently than conventional EVs and display stable structural integrity and antigen presentation compared to live DCs. Multi‐omic analysis …