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Accurate measurement of physical activity is fundamentally important in epidemiological research of physical activity behavior. A widely used telephone-based physical activity questionnaire was compared with other methods of administration and objective measures (pedometers and accelerometers) among 80 adults (43 women). The telephone questionnaire was comparable to both the self-administered form and international telephone-administered equivalent. Although moderate correlation coefficients with objective measures supported the use of the questionnaire, wide prediction intervals generated using Bland Altman methods highlighted large discrepancies between the measures, particularly in the moderate intensity category. These findings illustrate the limitations of correlation coefficients in validation studies and the inaccuracy of self-report questionnaires in measuring physical activity.