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Wound dressing should be impenetrable against microorganisms and it should keep the wound wet. Gauze and polyamide (PA) substrate were treated with various concentrations of AgNPs (25, 50, 75, and 100 ppm), PHMB (0.2, 0.4, 0.6, 0.8, and 1% w/v), and constant concentration of alginate (0.5% W/V) using a simple dipping method. Prepared samples were characterized by various techniques including Fourier transform infrared spectroscopy and scanning electron microscopy. The results indicated that the particles were successfully applied onto both substrates with an average diameter of particle size of 78 nm on gauze and 172 nm on the PA substrate surface (based on 50 nanoparticles). Antibacterial activity of the prepared nanocomposite against Staphylococcus aureus (gram-positive) bacteria on PA substrate and gauze were evaluated using the disc diffusion method. The results indicated that …