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Exosomes derived from adipose tissue‐derived mesenchymal stem cells (AD‐MSCs) have immunomodulatory effects of T‐cell inflammatory response and reduction of clinical symptoms on streptozotocin‐induced of the type‐1 diabetes mellitus (T1DM). Beside control group and untreated T1DM mice, a group of T1DM mice was treated with intraperitoneal injections of characterized exosomes derived from autologous AD‐MSCs. Body weight and blood glucose levels were measured during the procedure. Histopathology and immunohistochemistry were used for evaluation of pancreatic islets using hemotoxylin and eosin (H&E) staining and anti‐insulin antibody. Isolated splenic mononuclear cells (MNCs) were subjected to splenocytes proliferation assay using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, immunophenotyping of regulatory T cells and cytokines. A significant increase in the levels …