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Artemisia belongs to the family of Compositea; there are different Artemisias in Iran, of which Artemisia annua L. is grown in the north of Iran. In this study, Artemisinin was extracted and purified from the whole plants. The purification of Artemisinin was performed using column chromatography in different polarities of solvents and the results were evaluated by Thin Layer Chromatography (TLC). ¹H-NMR (NMR-500) spectroscopy was used to characterize the purified Artemisinin. The immunosuppressive activity of Artemisinin was investigated on Balb/c mice by DTH response in comparison to cyclosporin A (CsA). The data indicated that Artemisinin could suppress the delayed type hypersensitivity (DTH) against sheep blood capsule in Balb/c mice. Also its inhibitory effect on calmodulin (CaM) structure was determined by fluorescence spectroscopy. The data indicated an inhibitory effect of that on the activity of …