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A 107‐kD protein has been identified in primary cultures of chicken embryonic cardiomyocytes by immunoprecipitations with certain anti‐nebulin monoclonal antibodies (mAbs). These mAbs, prepared against a fragment of human skeletal muscle nebulin located near the carboxyl terminus, detect a 107‐kD protein in extracts of adult chicken heart, adult mouse heart, and adult rabbit heart by immunoblot analysis. A partial cDNA corresponding to this protein has been isolated by immunological screening of a chicken heart cDNA expression vector library. The partial cDNA encodes a 380‐amino acid open reading frame composed entirely of nebulin‐like 35‐residue modules marked by the highly conserved sequence motifs: SXXXYK and TPD. The open reading frame exhibits 60–85% homology with skeletal muscle nebulins from a variety of species. This cDNA recognizes an ˜8‐kb transcript in cardiac RNA and …