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There are a numbers of bioactive compounds in plants, such as alkaloids, tannins, flavonoids, sterols, triterpenes, etc., noted to have the major role in nutrition, physiology and control of diseases. Triterpenoids constitute one of the most characteristic classes of compounds in higher plants. The foremost important task in this paradigm is the screening of these compounds in the plants. The chromatographic study of the compounds serves to be a very useful and reliable source in the process of bioactive compounds screening in plants. According to the ethnobotanical information, it has been reported that the plant Lippia nodiflora possesses the antihypertensive potential. Hence in the present study, an attempt has been made to identify the phytochemical constituents of L. nodiflora using TLC and chemical derivatization method. Further, the isolation of the same compound is carried out by preparative HPTLC using the standardized solvent system viz., hexane: toluene: ethyl acetate (2: 15: 0.5). The confirmation of the isolation was done by GC-MS.