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This study explored the application of colloidal and immobilized silver nanoparticles (AgNPs) for inactivation of bacteriophages. Coliphages that are commonly used as indicators for enteric viruses, were used in this study. Colloidal AgNPs were synthesized via a chemical reduction approach using sodium borohydride as reducing agent and trisodium citrate as stabilizing agent. AgNP-immobilized glass substrate was prepared by immobilizing AgNPs on amine-functionalized glass substrate by post-immobilization method. The AgNP-immobilized glass substrate was also tested so as to minimize the release of AgNPs in the treated water. The characterization of AgNPs and the AgNP-immobilized glass surface was done using field emission gun-transmission electron microscopy and scanning electron microscopy. Studies conducted with varying concentrations of colloidal AgNPs displayed good antiviral activity for …