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Electrocardiogram (ECG) is an electric signal of cardiac activity posing highly discriminative properties related to human recognition. ECG based authentication has gained much success in recent times however discriminant feature extraction and efficient pattern classification still encounter numerous challenges. This paper proposed a novel methodology for ECG based biometric authentication system. Proposed method first denoise single lead raw ECG signal through empirical mode decomposition (EMD). Region of interest from ECG signals having maximum characteristic information related to subject's recognition is also extracted through EMD. Next, feature extraction is performed by combination of five features from statistical, time and frequency domains. Finally, selected features were categorized with range of different classification methods such as Support Vector Machines (SVM), K-nearest neighbor (KNN …