作者
Amrutlal K Patel, Ramesh J Pandit, Jalpa R Thakkar, Ankit T Hinsu, Vinod C Pandey, Joy K Pal, Kantilal S Prajapati, Subhash J Jakhesara, Chaitanya G Joshi
发表日期
2017/3/1
期刊
Veterinary research communications
卷号
41
期号
1
页码范围
67-75
出版商
Springer Netherlands
简介
Objective
Chicken astroviruses have been known to cause severe disease in chickens leading to increased mortality and “white chicks” condition. Here we aim to characterize the causative agent of visceral gout suspected for astrovirus infection in broiler breeder chickens.
Methods
Total RNA isolated from allantoic fluid of SPF embryo passaged with infected chicken sample was sequenced by whole genome shotgun sequencing using ion-torrent PGM platform. The sequence was analysed for the presence of coding and non-coding features, its similarity with reported isolates and epitope analysis of capsid structural protein.
Results
The consensus length of 7513 bp genome sequence of Indian isolate of chicken astrovirus was obtained after assembly of 14,121 high quality reads. The genome was comprised of 13 bp 5 …
学术搜索中的文章
AK Patel, RJ Pandit, JR Thakkar, AT Hinsu, VC Pandey… - Veterinary research communications, 2017