作者
Takanori Asakura, Kenichi Okuda, Gang Chen, Hong Dang, Takafumi Kato, Yu Mikami, Stephen A Schworer, Rodney C Gilmore, Giorgia Radicioni, Padraig Hawkins, Selene Margarita Barbosa Cardenas, Minako Saito, Anne Marie Cawley, Gabriela De la Cruz, Michael Chua, Neil E Alexis, Yohei Masugi, Peadar G Noone, Carla MP Ribeiro, Mehmet Kesimer, Kenneth N Olivier, Naoki Hasegawa, Scott H Randell, Wanda K O’Neal, Richard C Boucher
发表日期
2024/2/15
期刊
American journal of respiratory and critical care medicine
卷号
209
期号
4
页码范围
374-389
出版商
American Thoracic Society
简介
Rationale: Non–cystic fibrosis bronchiectasis (NCFB) may originate in bronchiolar regions of the lung. Accordingly, there is a need to characterize the morphology and molecular characteristics of NCFB bronchioles.
Objectives: Test the hypothesis that NCFB exhibits a major component of bronchiolar disease manifest by mucus plugging and ectasia.
Methods: Morphologic criteria and region-specific epithelial gene expression, measured histologically and by RNA in situ hybridization and immunohistochemistry, identified proximal and distal bronchioles in excised NCFB lungs. RNA in situ hybridization and immunohistochemistry assessed bronchiolar mucus accumulation and mucin gene expression. CRISPR-Cas9–mediated IL-1R1 knockout in human bronchial epithelial cultures tested IL-1α and IL-1β contributions to mucin production. Spatial transcriptional profiling characterized NCFB distal bronchiolar gene …
学术搜索中的文章
Proximal and Distal Bronchioles Contribute to the Pathogenesis of Non–Cystic Fibrosis Bronchiectasis
T Asakura, K Okuda, G Chen, H Dang, T Kato… - American journal of respiratory and critical care …, 2024