作者
Kenichi Okuda, Hong Dang, Yoshihiko Kobayashi, Gianni Carraro, Satoko Nakano, Gang Chen, Takafumi Kato, Takanori Asakura, Rodney C Gilmore, Lisa C Morton, Rhianna E Lee, Teresa Mascenik, Wei-Ning Yin, Selene Margarita Barbosa Cardenas, Yvonne K O’Neal, Caroline E Minnick, Michael Chua, Nancy L Quinney, Martina Gentzsch, Carlton W Anderson, Andrew Ghio, Hirotoshi Matsui, Takahide Nagase, Lawrence E Ostrowski, Barbara R Grubb, John C Olsen, Scott H Randell, Barry R Stripp, Purushothama Rao Tata, Wanda K O’Neal, Richard C Boucher
发表日期
2021/5/15
期刊
American journal of respiratory and critical care medicine
卷号
203
期号
10
页码范围
1275-1289
出版商
American Thoracic Society
简介
Rationale: Identification of the specific cell types expressing CFTR (cystic fibrosis [CF] transmembrane conductance regulator) is required for precision medicine therapies for CF. However, a full characterization of CFTR expression in normal human airway epithelia is missing.
Objectives: To identify the cell types that contribute to CFTR expression and function within the proximal–distal axis of the normal human lung.
Methods: Single-cell RNA (scRNA) sequencing (scRNA-seq) was performed on freshly isolated human large and small airway epithelial cells. scRNA in situ hybridization (ISH) and single-cell qRT-PCR were performed for validation. In vitro culture systems correlated CFTR function with cell types. Lentiviruses were used for cell type–specific transduction of wild-type CFTR in CF cells.
Measurements and Main Results: scRNA-seq identified secretory cells as dominating CFTR expression in normal human …
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K Okuda, H Dang, Y Kobayashi, G Carraro, S Nakano… - American journal of respiratory and critical care …, 2021