作者
Takafumi Kato, Takanori Asakura, Caitlin E Edwards, Hong Dang, Yu Mikami, Kenichi Okuda, Gang Chen, Ling Sun, Rodney C Gilmore, Padraig Hawkins, Gabriela De la Cruz, Michelle R Cooley, Alexis B Bailey, Stephen M Hewitt, Daniel S Chertow, Alain C Borczuk, Steven Salvatore, Fernando J Martinez, Leigh B Thorne, Frederic B Askin, Camille Ehre, Scott H Randell, Wanda K O’Neal, Ralph S Baric, Richard C Boucher
发表日期
2022/12/1
期刊
American journal of respiratory and critical care medicine
卷号
206
期号
11
页码范围
1336-1352
出版商
American Thoracic Society
简介
Rationale: The incidence and sites of mucus accumulation and molecular regulation of mucin gene expression in coronavirus (COVID-19) lung disease have not been reported.
Objectives: To characterize the incidence of mucus accumulation and the mechanisms mediating mucin hypersecretion in COVID-19 lung disease.
Methods: Airway mucus and mucins were evaluated in COVID-19 autopsy lungs by Alcian blue and periodic acid–Schiff staining, immunohistochemical staining, RNA in situ hybridization, and spatial transcriptional profiling. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected human bronchial epithelial (HBE) cultures were used to investigate mechanisms of SARS-CoV-2–induced mucin expression and synthesis and test candidate countermeasures.
Measurements and Main Results: MUC5B and variably MUC5AC RNA concentrations were increased throughout all airway …
学术搜索中的文章
T Kato, T Asakura, CE Edwards, H Dang, Y Mikami… - American journal of respiratory and critical care …, 2022