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The European Union (EU) has set a binding greenhouse gas (GHG) emission reduction target for transportation biofuels and other bioliquids. In this study, the GHG emissions of various biofuel chains considered as relevant in large-scale production in Finland were calculated in accordance with the EU sustainability criteria. Special attention was paid to uncertainties and the sensitivities of certain parameters. According to the results, it is impossible in many cases to unambiguously conclude whether or not a biofuel chain passes the emission-saving limit provided by the EU. This may reduce the willingness to invest in biofuel production. Major sources of uncertainties and sensitivities are nitrous oxide emissions from soil and nitrogen fertilisation, emissions of process heat production and soil carbon stock changes in biomass production. Several propositions are made in order to reduce the uncertainty of the results …