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The leaf of nicotiana tabacum was collected, gabbled, pulverized air dried and subjected to gradient extraction with soxhlet apparatus and the different extracts were screened phytochemically for its chemical components. This revealed the presence of alkaloids, reducing compounds (carbohydrates),, flavonoids, saponins, terpenes and steroids in moderate concentration. hptlc fingerprintint parameters had been developed for alcoholic leaf extract of nicotiana tabacum. At wavelength (550 nm), resolution was better for this extracts and hence, this wavelength can be taken for obtaining optimum hptlc fingerprinting for this medicinal plant.