作者
Miguel A Martín-Acebes, Angela Vázquez-Calvo, Verónica Rincón, Mauricio G Mateu, Francisco Sobrino
发表日期
2011/3/15
期刊
Journal of Virology
卷号
85
期号
6
页码范围
2733-2740
出版商
American Society for Microbiology
简介
Foot-and-mouth disease virus (FMDV) particles lose infectivity due to their disassembly at pH values slightly below neutrality. This acid-dependent disassembly process is required for viral RNA release inside endosomes. To study the molecular determinants of viral resistance to acid-induced disassembly, six FMDV variants with increased resistance to acid inactivation were isolated. Infection by these mutants was more sensitive to drugs that raise the endosomal pH (NH4Cl and concanamycin A) than was infection by the parental C-S8c1 virus, confirming that the increase in acid resistance is related to a lower pH requirement for productive uncoating. Amino acid replacement N17D at the N terminus of VP1 capsid protein was found in all six mutants. This single substitution was shown to be responsible for increased acid resistance when introduced into an infectious FMDV clone. The increased resistance of this …
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MA Martín-Acebes, A Vázquez-Calvo, V Rincón… - Journal of Virology, 2011