作者
Lili Li, Meiling Gao, Peng Jiao, Shulong Zu, Yong-qiang Deng, Dingyi Wan, Yang Cao, Jing Duan, Saba R Aliyari, Jie Li, Yueyue Shi, Zihe Rao, Cheng-feng Qin, Yu Guo, Genhong Cheng, Heng Yang
发表日期
2022/5/17
期刊
Cell & Bioscience
卷号
12
期号
1
页码范围
63
出版商
BioMed Central
简介
Background
Neutralizing antibodies are approved drugs to treat coronavirus disease-2019 (COVID-19) patients, yet mutations in severe acute respiratory syndrome coronavirus (SARS-CoV-2) variants may reduce the antibody neutralizing activity. New monoclonal antibodies (mAbs) and antibody remolding strategies are recalled in the battle with COVID-19 epidemic.
Results
We identified multiple mAbs from antibody phage display library made from COVID-19 patients and further characterized the R3P1-E4 clone, which effectively suppressed SARS-CoV-2 infection and rescued the lethal phenotype in mice infected with SARS-CoV-2. Crystal structural analysis not only explained why R3P1-E4 had selectively reduced binding and neutralizing activity to SARS-CoV-2 variants carrying K417 mutations, but also allowed us to engineer mutant antibodies with improved neutralizing activity against these variants. Thus, we …
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L Li, M Gao, P Jiao, S Zu, Y Deng, D Wan, Y Cao… - Cell & Bioscience, 2022